Australian Genome Research Facility Ltd (AGRF)

Profile

The Australian Genome Research Facility Ltd (AGRF) is a not for profit organization. It is Australia’s largest genomic service provider to academia and industry, currently serving over 3,000 clients nationally. Each AGRF node provides a gateway to the AGRF network of state-of-the-art facilities, technology and expertise.

AGRF is accredited by NATA to the ISO/IEC17025:2005 Quality Standard and aims to continually look at the development and introduction of new services as technology and demand evolves. AGRF receives Federal Government funding support through the NCRIS program and Bioplatforms Australia.

 

Description

We believe in improving the quality of life through exceptional life science. Our mission is to empower Australia to be recognised for world-class innovation and leading edge genomics research.

We are a not-for-profit organisation, and through a national network we provide leading edge services and expertise, easily accessible at the local level.

By exchanging and collaborating in knowledge expertise, providing access to innovative and state-of-the-art genomic technologies, we enable Australian researchers to advance leading edge genomics internationally.

We assist Australia’s access to the latest genomic technologies to advance national genomics research.

 

Products and services

As part of the AGRF network, the Adelaide Node currently focuses on:

  • Next Generation and Sanger DNA sequencing
  • DNA extraction from a wide variety of sample types
  • Plant growth and stress
  • Genetic testing for quality control in industry

 

Type Manufacturer Model Technical Specifications
Genomics
DNA Analyser
Next Generation Sequencing
illumina NextSeq500  
DNA Analyser
Next Generation Sequencing
illumina MiSeq  
DNA Analyser
Next Generation Sequencing
Thermo Fisher Ion Torrent PGM  
DNA Analyser
Fluorescence based
Thermo Fisher AB 3730 & AB 3730xl  
Library Preparation Thermo Fisher One Touch ES  
DNA re-purification Boreal Genomics Aurora  
Genogrinder / Retch mills Qiagen Retch mills Genomic DNA Purification
Liquid handlers Eppendorf epMotion 5075  
-20 C and -80 C freezer space Sanyo MDF-U73V  
PCR Blocks, 96 well format Thermofisher Veriti (x 6)  
Sonikation Covaris Covaris S2  
Spectrometry
Dual-scanning microplate spectrofluorimeter Molecular Dynamics SPECTRAmax GEMINI XPS  
HTP UV Spectrophotometry  Trinean / Thermo Fisher Dropsense 96 / Nanodrop ND8000  
Name Description
Genomics
DNA Sequencing AGRF provides a wide range of DNA sequencing options, including shotgun and amplicon based library preparation, Next Generation and Sanger sequencing. Local services are based on illumina MiSeq and NextSeq500 systems, and AB3730/3730xl capillary analysers. Larger projects are transferred to our Melbourne or Brisbane nodes, with Pyrosequencing conducted at our Perth facility.
Genomic DNA extraction A range of options exist for generation of genomic DNA from fresh or frozen specimens.
Genotyping A comprehensive range of genotyping options and platforms are available. These include illumina iScan, Sequenom, Pyromark and Applied Biosystems platforms.
Bioinformatics AGRF has a team of Bioinformaticians available to assist in project design and data handling.

Contact

Dr John Stephen

National Operations Manager
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Location

Adelaide Node
Plant Genomics Centre
The University of Adelaide
Waite Campus
Urrbrae SA 5064