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Cell Screen SA (CeSSA) provides automated liquid handling, high-content imaging, measurement of fluorescence-based assays and measurement of luminescence-based assays.

CeSSA is located in the Flinders Centre for Innovation in Cancer. The instrumentation in the laboratory can be used for single plate/sample assays through to high-throughput screening (up to 110 x 384 well plates, without manual handling, depending on assay) and is focused around several Perkin Elmer instruments:

  • Janus workstation for automated pipetting
  • Operetta microscope for high-content imaging and analysis
  • Ensight for plate-based luminescent and fluorescent assays
  • plate::handler II for plate movement

We also have:

  • a BioTek 406 washer for automated plate washing and pipetting of inexpensive reagents
  • Liconic heat and CO2 controlled incubator
  • Liconic room temperature plate stacker

Integration of all instruments with the plate::handler allows for complete automation of plate movement between instruments.

Instruments can also be used separately.

 

Products and services

CeSSA instrumentation can be used for:

  • High-throughput screening of nucleic acid and chemical libraries
  • Automated pipetting procedures, including: labware (tube etc) to plate, sample dilutions, plate treatments, cell seeding
  • High-content imaging using brightfield, confocal or non-confocal fluorescence, as well as image analysis
  • Absorbance, fluorescence, luminescence, time-resolved fluorescence and Alpha-assay plate reading
  • Automated workflows combining all or some instruments, for example time-course imaging

 

 

Type Manufacturer Model Technical Specifications
Automated liquid handling Perkin Elmer Janus Standard/mid-size deck. 8-tip Varispan arm. MDT arm with P50 and P235 disposable tip heads.
Plate reader Perkin Elmer Ensight Absorbance, luminescence, fluorescence (top and bottom reading), laser-based Alpha, time-resolved fluorescence, temperature controlled. Kaleido software.
High-content Microscope Perkin Elmer Operetta Spinning disc confocal or non-confocal fluorescence. Brightfield. Harmony software.
Plate washer BioTek 406 Aspiration and dispensing. 96-well and 384-well plates
Automated plate movements Perkin Elmer plate:handler II  
Incubator & Plate Hotel Liconic   Capacity 110 plates
Cell counter Olympus R1

Cell counts in 10 – 15 seconds, cell concentration range 5×104 – 1×107 cells/mL, counts cells 3 – 60µm in size.
Reports total, live, dead cell concentration and number; viability; average cell size; histograms of cell size distribution

Name Description
Chemical and nucleic acid library screening <5000 data point library screening. We can help with library selection.
High-content imaging Brightfield, digital phase contrast, confocal/non-confocal, time-lapse, CO2 controlled stage, simplified, automated image analysis using Harmony software. Slides to 384-well plates. Optimised for plates.
Plate reading Plate-based assays with fluorescence, time-resolved fluorescence, luminescence, absorbance read-out. Alpha-based assays.
Automated pipetting plate-to-plate, well-to-well. 96 and 384 well plates. Labware (dish/tube)-to-plate, labware-to-labware.
Fully automated workflows between instruments Automated plate movement between Ensight, Operetta, Janus, washer, plate stacker, and incubator. E.g. automated time-lapse imaging.

 

Contact

Dr Amanda Aloia

Facility Manager
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Location

Room 4L172
Flinders Centre for Innovation in Cancer
Flinders Drive
Bedford Park SA 5042